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- Cadherin
細胞接着分子カドヘリンの研究
(Research on Cell Adhesion Molecule "Cadherin")
細胞接着分子(Cell Adhesion Molecules)
カドヘリン発見物語(理研竹市研)(Discovery of Cadherin)
カドヘリンの発見
竹市は京大の岡田研に戻り、カルシウム依存的な細胞接着分子の同定するために、細胞間接着を阻害する抗体の作成を試みました。当時、テラトカシノーマ・F9細胞に対する抗体が8細胞期のマウス胚のコンパクションを阻害することが報告されました(Kemler et al., 1977)。この現象と自分が見ている細胞間接着の現象は似ていると直感した竹市は、F9細胞のポリクローナル抗体を作成し、それが細胞接着を阻害できることを発見しました(Yoshida-Noro and Takeichi, 1982)。さらに、細胞接着を阻害できるモノクロナール抗体のクローニングに成功しました(Yoshida-Noro et al., 1984)。
細胞から個体へ − 脇道から到達した発生生物学の本流(JT生命誌研究館)(竹市)(Naming of Cadehrin)
この大事な接着分子に名前を早く付けろと岡田先生に急かされて、この分子の研究に携わった野呂知加子さん(現日本大学助教授)達と相談して、カルシウム(calcium)と接着(adhere)の2つの単語を合わせたカドヘリン(cadherin)に決めました。
Discovery and characterization of the cadherin family of cell adhesion molecules. An interview with Masatoshi Takeichi.
Douglas Sipp
Int. J. Dev. Biol. (2004) 48: 387-396
How did you settle on the name “cadherin?”
I had a few ideas for names, but I wasn’t confident about what would sound good in English. There was a researcher named David de Pomerai on a one-month visit to the Okada lab at the time, so I asked him his opinion and he said cadherin worked well. Actually, it was Chikako Yoshida who first proposed ‘cadherin,’ combining elements from ‘calcium’ and ‘adhere’.
トムソン・ロイター引用栄誉賞『細胞接着分子カドヘリンの発見』 2012(竹市)細胞のメカニズム解明から医療へ。発生・再生科学の今(Thomson Reuters)
Cadherinに関する自分の論文(My Papers)
- Dev Biol. 1981 Oct 30;87(2):340-50.
Selective adhesion of embryonal carcinoma cells and differentiated cells by Ca2+-dependent sites.
Takeichi M, Atsumi T, Yoshida C, Uno K, Okada TS.
PMID: 6793433 - Cell. 1982 Feb;28(2):217-24.
Teratocarcinoma cell adhesion: identification of a cell-surface protein involved in calcium-dependent cell aggregation.
Yoshida C, Takeichi M.
PMID: 7060128 - J Cell Biol. 1983 Sep;97(3):944-8.
Calcium-dependent cell-cell adhesion molecules common to hepatocytes and teratocarcinoma stem cells.
Ogou SI, Yoshida-Noro C, Takeichi M.
PMID: 6885928 - Dev Biol. 1984 Jan;101(1):19-27.
Molecular nature of the calcium-dependent cell-cell adhesion system in mouse teratocarcinoma and embryonic cells studied with a monoclonal antibody.
Yoshida-Noro C, Suzuki N, Takeichi M.
PMID: 6692973
We, thus, propose to term the molecules recognized by ECCD-1, “cadherin.” The major antigen recognized with ECCD-1 will be specified as CAD-124. (in Discussion) - Exp Cell Res. 1984 May;152(1):270-4.
Monoclonal antibody ECCD-1 inhibits intercellular communication in teratocarcinoma PCC3 cells.
Kanno Y, Sasaki Y, Shiba Y, Yoshida-Noro C, Takeichi M.
PMID: 6714323 - Develop. Growth Differ. 1985Jul; 27(6), 673-678
Detection of the initial step of mesenchymal differentiation of teratocarcinoma cells using the monoclonal antibody ECCD-1.
Yoshida-Noro C, Takeichi M, Okada TS - Development. 1994 Jan;120(1):49-57.
A functional test for maternally inherited cadherin in Xenopus shows its importance in cell adhesion at the blastula stage.
Heasman J, Ginsberg D, Geiger B, Goldstone K, Pratt T, Yoshida-Noro C, Wylie C.
PMID: 8119131 - Cell. 1994 Dec 2;79(5):791-803.
Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos.
Heasman J1, Crawford A, Goldstone K, Garner-Hamrick P, Gumbiner B, McCrea P, Kintner C, Noro CY, Wylie C.
PMID: 7528101